Trypsin Inhibitor Antikörper

Produktnummer ABIN107871

Hersteller Produkt- Nr.: 200-401-079

Dieser Anti-Trypsin Inhibitor Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von Trypsin Inhibitor in WB, ELISA und IP. Geeignet für Soybean.

Kurzübersicht für Trypsin Inhibitor Antikörper (ABIN107871)

Target: Trypsin Inhibitor

Reaktivität: Soybean

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Trypsin Inhibitor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-Trypsin Inhibitor Antikörper

Hersteller Produkt- Nr.: 200-401-079

Hersteller: Rockland

Verwendungszweck: Trypsin Inhibitor Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soybean].

Produktmerkmale: Synonyms: rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Aufreinigung: Anti-trypsin inhibitor antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Trypsin Inhibitor [Soybean]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Soybean trypsin inhibitor antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Trypsin Inhibitor [Soybean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Trypsin Inhibitor

Target: Trypsin Inhibitor

Hintergrund: Background: Trypsin inhibitor antibody is specific for soybean trypsin inhibitor. Soybean trypsin inhibitor inhibits trypsin, but also chymotrypsin and plasmin at a reduced level. SBTI has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. Soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases.

Gen-ID: 547831

UniProt: P01070